Protocols

***Please contact the lab to see protocols 

• Calcineurin phosphatase activity assay for Cn inhibitor screen  
  
• Collecting and processing mouse bronchoalveolar lavage
• Confirming the engraftment from BM chimera by staining Peripheral Blood Mononuclear Cell (PBMC) with CD45.1 versus CD45.2
• Congenic Bone Marrow Transplant protocol_UBC-CreERT2/CnB1f/f strain
• Extracting RNA from bronchoalveolar lavage (BAL) cell pellet for qPCR
• ImageJ threshold edema analysis for pancreatic H&E slides
• Immunohistochemistry_Paraffin-embedded Blocks
• Isolating primary mouse pancreatic acinar cells for platereader-based assays or confocal live cell imaging
• L-arginine model
• Measuring Serum Amylase using Phadebas kit
• Version 1_NFAT activity assay using Ad-NFAT-luciferase
• Pancreatic tissue collection for high quality RNA extraction
• PI uptake
• Prepare Splenocytes and Bone Marrow cells for DNA and RNA and Protein
• Purifying pancreatic ductal cell by FACS sorting DBA+ cells
• RNA extraction using TRIzol homogenates
• Setting up qPCR reaction in a 96-well plate
• Pancreatic Cell Lysate for Western Blotting
• Western Blot Protocol
• Mouse Islet Isolation
• Glucose Stimulated Insulin Secretion