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THE HUSAIN LAB
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Protocols

***Please contact the lab to see protocols 
  • Calcineurin phosphatase activity assay for Cn inhibitor screen  
  • Collecting and processing mouse bronchoalveolar lavage
  • Confirming the engraftment from BM chimera by staining Peripheral Blood Mononuclear Cell (PBMC) with CD45.1 versus CD45.2
  • Congenic Bone Marrow Transplant protocol_UBC-CreERT2/CnB1f/f strain
  • Extracting RNA from bronchoalveolar lavage (BAL) cell pellet for qPCR
  • ImageJ threshold edema analysis for pancreatic H&E slides
  • Immunohistochemistry_Paraffin-embedded Blocks
  • Isolating primary mouse pancreatic acinar cells for platereader-based assays or confocal live cell imaging
  • L-arginine model
  • Measuring Serum Amylase using Phadebas kit
  • Version 1_NFAT activity assay using Ad-NFAT-luciferase
  • Pancreatic tissue collection for high quality RNA extraction
  • PI uptake
  • Prepare Splenocytes and Bone Marrow cells for DNA and RNA and Protein
  • Purifying pancreatic ductal cell by FACS sorting DBA+ cells
  • RNA extraction using TRIzol homogenates
  • Setting up qPCR reaction in a 96-well plate
  • Pancreatic Cell Lysate for Western Blotting
  • Western Blot Protocol
  • Mouse Islet Isolation
  • Glucose Stimulated Insulin Secretion
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    • Join Our Team